Abstract
AbstractSeparator isoelectric focusing is a simple technique for creating narrow pH gradients (less than 1 pH unit wide). It has proved useful elsewhere for the enhanced separation of phosphoglucomutase, esterase D, group specific component (Gc) haemoglobin, transferrin and for distinguishing between seminal and vaginal acid phosphatases. The properties of 10 different zwitterionic buffers have been studied in order to determine pH gradient profiles, their stability, experimental reproducibility and the effect of temperature. The effect of adding non‐ionic constituents (glycerol or sucrose) to the gel has also been investigated. The buffers examined produced narrow pH gradients within the pH 4–6 range. It was shown that specific pH gradients could be produced by mixing different buffers and carrier ampholytes together.
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