Abstract
Summary Seed germination pouches or seedling growth pouches have been utilised previously for high throughput resistance screening of root-knot nematodes, Meloidogyne spp., on legume plants. In this study we demonstrated that soybean cyst nematode (SCN) Heterodera glycines, with a different reproduction mode from root-knot nematode, is also able to invade, complete its life cycle and reproduce in soybean roots in seedling pouches similar to the pot system with soil, indicating seedling pouches can be utilised as an alternate SCN culture method when glasshouse/growth chamber space is limited. Moreover, seedling pouches provide an easy approach to collect large numbers of clean male nematodes, which could accelerate SCN biological study such as SCN mating behaviour and molecular signals associated with mating. The results of SCN resistance screening indicate that seedling pouches could be utilised for initial resistance screening with SCN.
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