Application of SAT technique in detection of Chlamydia trachomatis and Ureaplasma urealyticum

Abstract

Objective To study the Chlamydia trachomatis (CT) and Ureaplasma urealyticum (UU) infection in Guangzhou area, and analyze the consistency of simultaneous amplification and testing (SAT) and conventional methods (CT was detected by latex immunochromatography, UU was detected by liquid culture method). Methods A total of 12 120 samples of urogenital secretions or urine samples were collected from Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University from January 2015 to December 2016. CT-RNA and UU-RNA were detected by the SAT technique, a part of samples were tested by conventional methods at the same time. The positive rates of CT and UU by SAT and the conventional methods between different gender and age groups were analyzed by χ2 test, the consistencies between different detection methods were analyzed by Kappa test. Results The positive rate of CT was 4.05%(356/8 781), UU 33.69%(1 125/3 339)in Guangzhou from 2015 to 2016. The positive rate of UU was significantly higher than that of CT (χ2=1 981, P<0.01). Of 145 specimens for CT test, the coincidence rate between SAT and latex immunochromatographic method was 96.55%(140/145), which showed good consistency (Kappa=0.65). Of 186 specimens for UU test, the coincidence rate of the results between the SAT method and liquid culture was 92.47%(172/186), which showed strong consistency (Kappa=0.81). Conclusions The positive rate of UU was significantly higher than that of CT in Guangzhou. The SAT method and conventional methods to detect CT and UU show high consistency, which can provide the evidence for clinical diagnosis of CT and UU infection.(Chin J Lab Med, 2018, 41: 380-384) Key words: Chlamydia trachomatis; Ureaplasma urealyticum; Bacteriological techniques; Nucleic acid amplification techniques; Immunochromatography