Application of reversed phase chromatorotorTM for the isolation of natural products by spin chromatographic system

Abstract

A new technology for the preparation of ChromatorotorTM (WO 2013/036803 A8) with a choice of chromatographic phases for Centrifugal Preparative Thin-layer Chromatography (CPTLC) or Rotational Planar Chromatography (RPC) opened a new window for fast, reliable, and economic separations of various classes of natural products. In order to prepare ChromatorotorsTM, together with the separation of compounds in the same instrument, an improved version of CPTLC, named Spin Cheromatography System (UM 8390), was developed. Polar and/or semi-polar compounds with close Rf values, as well as extracts and column fractions were separated and/or purified in a preparative and/or semi-preparative scale using the C18 silica gel rotors in RP mode, eluted with mixtures of aqueous-based solvents. Three model examples of this application, using RP ChromatorotorsTM, for the isolation of the diastereoisomeric alkaloids banistenosides I and II from Banisteriopsis caapi, saponins III and IV from Fagonia cretica, and the sesquiterpenes artemisinin (V) and artemisinic acid (VI) from Artemisia annua.