Abstract

In the analysis of testosterone propionate in oil injectables, reverse phase chromatography on silanized purified siliceous earth provides a convenient method for separating testosterone propionate from the bulk of the oil. The latter is retained on the column, while the steroid, together with the sterol and triterpenoid components of the oil, is eluted. The testosterone propionate is quantitatively separated from this mixture by direct phase chromatography, with nitromethane as immobile phase and heptane as eluant; the testosterone propionate is then determined spectrophotometrically.

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