Application of response surface protein hydrolysis process crucian carp

Abstract

This experiment mainly the degree of hydrolysis as an index, considering the amount of enzyme, substrate concentration, temperature and PH value on the influencing factors, through the response surface method for screening protein, alkaline protease hydrolysis crucian carp optimum conditions. Experimental results show that the amount of enzyme (E / S) 1.5%, the substrate concentration of 1%, temperature 45 °C pH value of 9.5. The higher the degree of hydrolysis of 12.31%.