Abstract
AbstractResponse surface methodology (RSM) was applied to optimize the conditions of nitrate removal by Pseudomonas strain AN‐1 at low temperature, which was isolated from aniline‐contaminated groundwater. A three‐factor, three‐level Box–Behnken experimental design and analysis of variance were combined with the obtained second‐order polynomial equation and applied for RSM optimization. By solving the second‐order polynomial equation and analysing the 3D response surface, a ‐N concentration of 65.29 mg/L, an initial pH of 6.65, and an initial DO of 7.53 were identified as the optimized conditions. These conditions achieved a removal percentage of 53.7485% after 48 h at 10°C, which was in good agreement with the predicted 53.8085%. This study showed the excellent ability of the Pseudomonas strain AN‐1 to degrade nitrate at low temperatures, which provided a solid foundation for the further application of this microorganism in the treatment of nitrate‐contaminated groundwater.
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