Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460

Taswar Ahsan,Yuanhua Wu,Muhammad Irfan,Jianguang Chen

doi:10.1186/s13568-017-0388-z

Taswar Ahsan, Yuanhua Wu + Show 2 more

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https://doi.org/10.1186/s13568-017-0388-z

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Abstract

A bioactive strain Streptomyces diastatochromogenes KX852460 was selected for the production of secondary metabolites to control the target spot disease on tobacco leaves, caused by the Rhizoctonia solani AG-3. Peanut meal, soluble starch, NaCl, yeast extract, and ammonium sulphate were identified the best ingredient for high antifungal activity of S. diastatochromogenes KX852460 against the R. solani AG-3. For the improved production of secondary metabolites, central composite design of response surface methodology was applied in submerged fermentation. The best concentrations of ingredients were peanut meal 4.88%, soluble starch 4.40%, NaCl 0.52%, yeast extract 0.47%, and ammonium sulphate 0.0360%. Study of metabolism changes in the submerged fermentation process was analyzed. Level of the reducing sugar increased, as the total sugar consumed. Amino nitrogen and total sugar decrease tendency, which indicated the growth of bacteria in submerged fermentation batch. Production of secondary and other metabolites influenced the pH of the fermentation batch.

Highlights

Rhizoctonia solani is the major soil borne pathogen and the causal agent of several kinds of diseases in plants throughout the world, and have destructive impacts on the production of tobacco
Nitrogen and microelement sources on antifungal activity of Strain KX852460 Peanut meal, wheat bran, soya bean meal and soluble starch were used as carbon and nitrogen source
Calcium carbonate and magnesium sulphate had no significant effects on the antifungal activity against R. solani AG-3 by the strain KX852460 (Fig. 3b, d)

Summary

Introduction

Rhizoctonia solani is the major soil borne pathogen and the causal agent of several kinds of diseases in plants throughout the world, and have destructive impacts on the production of tobacco. In the Liaoning province of China in 2006 target spot disease on tobacco leaves was investigated and analyzed that R. solani AG-3 was the causal agent of this disease (Wu et al 2012). Medium composition and fermentation conditions may cause effects on the production of secondary metabolites from the Streptomyces. In the yield of secondary metabolites these parameters have a key role (Al-Hulu 2013). By fermentation production of enzymes and growth of microbes, directly related to the medium composition and conditions. Optimization of these parameters became a significant process. To optimize these parameters for the fermentation, several statics approaches are widely utilized (Kumar et al 2016)

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