Abstract
Ratio derivative spectrophotometrie method has been developed for the simultaneous determination of naphazoline (NAP) and antazoline (ANT) at micromolar levels in Britton Robinson buffer (pH 9) medium. In this method the overlapping spectra of naphazoline and antazoline were well resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbances of naphazoline and antazoline were measured at 227.2 and 235 nm, respectively for their quantification. The method is simple, fast and does not require separation of naphazoline and antazoline. Another salient feature of the method is that simultaneous standard additions of both analytes permitted to resolve matrix effect and quantification at a unique standard addition plot. Naphazoline and antazoline were determined in the concentration range of 10-150 μmol L-1(NAP/ANT ratio varying from about 10 to 150) in the same aliquot with a precision and accuracy of about 1.7% and 1.8%, respectively. The recommended procedure was successfully applied for analysis of naphazoline and antazoline in eye drops.
Highlights
Naphazoline hydrochloride (NAP) [2-(1-naphthylmethyl)-2-imidazoline monohydrochloride] is a sympathomimetic, which belongs to the imidazole group
This report describes a simple, sensitive and rapid procedure for simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops based on derivative spectrophotometry
The combination of ratio derivative spectrophotometry and standard addition methods was used for resolving of additive interference and matrix effect simultaneously
Summary
Naphazoline hydrochloride (NAP) [2-(1-naphthylmethyl)-2-imidazoline monohydrochloride] is a sympathomimetic, which belongs to the imidazole group. Gas chromatographic and high performance liquid chromatographic methods were reported for determination of naphazoline and antazoline in pharmaceutical preparations[12,13,14]. These methods are complicated and need the expensive instruments. This report describes a simple, sensitive and rapid procedure for simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops based on derivative spectrophotometry. Principal component and partial least squares solutions require no explicit data about the individual interferences to be known in order to model and to eliminate them[17] They require a set of calibration samples with known concentrations of the analyte to be determined and the concentration of each interferent in different samples to be varied to allow the calibration algorithm to model its effect. A pH meter (Metrohm, Model 691) with a double junction glass electrode was used to check the pH of the solutions
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