Abstract
In this study, the RAPD (Random Amplified Polymorphic DNA) technology was used to identify the genotypic variation between the parental strain and the shuffled strain of Streptomyces gilvosporeus. Firstly, RAPD conditions were optimized by orthogonal test. The results were as follows: DNA 60-150 ng, Taq DNA polymerase 1.0-1.5U, primer concentration 0.3-0.4 mmol/L, dNTPs concentration 200-250 μmol/L, Mg <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2+</sup> concentration 2.5-3.0 mmol/L in 20 μL PCR system. Under above optimal conditions, abundant, stable and clear strips could be obtained and the primer S1458 with good repeatability and polymorphism was further screened out. It was found that the specific fragment about 1500 bp was observed in shuffled strain and partial sequence band had a high homology with the MarR-family transcription regulator factors existing in several streptomyces. The results indicated that RAPD technology was contribute to determining genotypic differences in Streptomyces gilvosporeus strains from genome shuffling and serving as an effective tool for exploring the biosynthetic genes correlated with metabolic product.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
