Abstract
Abstract Progress curve analysis has been tested as a method of estimating enzyme kinetic parameters when enzyme activity must be determined by using radiolabeled substrates. The initial concentrations of adenosine have been selected in order to achieve the maximum absolute sensitivity of adenosine with respect to the parameters of the equation. The results obtained show that this method gives the same results as initial-rate analysts and has the advantages that it is faster and cheaper. Kinetic parameter estimates of commercial adenosine deaminase, when [3H] adenosine is used as a substrate, are determined in order to prove the applicability of the procedure.
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