Abstract
Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column labeling technique for analysis of canine PFAA levels. This method significantly shortened the analysis time relative to conventional methods. No diurnal fluctuations were detected at 9:00 AM in most PFAA levels, and food intake increased the levels of some PFAAs, including valine, leucine, tyrosine, phenylalanine, and proline. These results indicate that LC with pre-column labeling is useful for measuring canine PFAA levels, for which time of day and interval after food intake must be taken into consideration.
Highlights
Amino acids (AAs) are important substrates for and regulators of metabolic pathways [1]
We found that plasma EAA levels were increased after food intake, with changes in Tyr, Phe, and Pro levels observed within 8 h
These results indicate that food intake must be considered for blood collection in analysis of canine Plasma-free amino acid (PFAA) levels
Summary
Amino acids (AAs) are important substrates for and regulators of metabolic pathways [1]. A link has been reported between canine cancer and PFAA levels; plasma levels of glutamine (Gln), serine (Ser), asparagine (Asn), and alanine (Ala) were lower in dogs with malignant mammary gland tumors than in healthy animals [6], while plasma levels of threonine (Thr), proline (Pro), and Ser were reduced in canine oral malignant melanoma relative to controls [7]. PFAA levels in humans are influenced by many factors, including diurnal and hormonal fluctuations and the food ingested [10,11,12,13]. PFAA the levels in humans are influenced byand many factors, diurnal hormonal describing effects of diurnal fluctuations food intakeincluding on canine. Analysis and investigate the effects of diurnal fluctuations and food intake which affect PFAA levels
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
