Abstract
The diagnostic feature of a neuroendocrine tumor by transmission electron microscopy (TEM) is the presence of neurosecretory granules (NSG). However, other electron dense inclusions like lipid droplets and primary lysosomes may mimic NSC. In this study, post-embedding immunogold (PEI) technique using chromogranin A antibody has been used to confirm the ultrastructural impression of neuroendocrine tumor. This technique is contrasted with immunohistochemical results.Material for this study was obtained from 5 transabdominal fine needle aspirates of multiple hepatic masses of unknown nature, processed for light and electron microscopic examinations and immunohistochemistry. Thin gold sections were cut (Reichart) with a Diatome diamond knife and mounted on 200 mesh nickel grids. A nickel grid with section side down was placed on drops of 11% aqueous sodium metaperiodate for 30 minutes, and was then washed with distilled water. Grids were incubated in 1% albumin for 15 minutes and transferred directly to chromogranin A and incubated for 60 minutes at room temperature.
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