Abstract
This review focuses on the use of conducting polymer (CP) films in electrochemical affinity sensors and emphasizes innovative designs and unique applications of immunosensors. The review covers some aspects in the application of polypyrrole (Ppy) for the creation of immunosensors. Polypyrrole film fabrication methods like solvent casting, adsorption, and electropolymerization are presented. The focus, is on electrochemically synthesized Ppy as very promising material for the formation of miniaturized electrochemical immunosensors. Polypyrrole films implemented in various capacities in amperometric, conductometric, and potentiometric immunosensor design are reviewed. The acceptance of immobilization and detection approaches used recently in affinity sensors with critical analysis applied in certain techniques is discussed. The biologically active components (BAC) used for the creation of polypyrrole-based immunosensors are described briefly. Some future trends in the development of polypyrrole-based immunosensors are predicted, as well as possible directions discussed.
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