Abstract
Two bovine meat samples were analysed although the samples were already in a state of advanced decomposition. The isolated DNA was extremely degraded and not suitable for conventional DNA fingerprinting (profiling), thus the polymerase chain reaction (PCR) was applied to screen for a Y-chromosome specific fragment and the loci of the two milk proteins κ-casein and β-lactoglobulin. Both samples contained Y-chromosome specific DNA and the κ-casein genotype AA but they differed at the β-lactoglobulin locus (BB versus AB). Thus a different origin (carcass) could be verified for the two samples and a subject was exonerated from a case of meat larceny.
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