Abstract
Abstract—Neutrophil extracellular traps (NET) play an important role in a body’s antimicrobial defense. However, excessive formation leads to various pathological processes in the body. Therefore, a quantitative assessment of the NET content in human whole blood is very important in clinical practice. An approach is proposed allowing the use of polymer microspheres (PMS), the surfaces of which are modified with heparin as sorbents for isolation from whole blood and quantitative determination of NET. To solve this problem, polystyrene microspheres with an average diameter of 140 μm were synthesized, which contain amino groups; covalent immobilization of heparin on their surface was carried out, and a close to linear relationship between the amount of NET and DNA-histone complexes binding to the surface of the synthesized PMS was demonstrated. The data indicate that the approach may be promising both in basic research and clinical practice.
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