Application of Platelet-rich Plasma (PRP) with Different Scaffolds in Tissue Engineering

Abstract

Background: Today, stem cells are the best candidates for cell therapy and tissue engineering. Adipose-derived Stem Cells (ADSCs) are an essential source of cells in replacement therapies of many diseases. Objectives: This study compared the proliferation of ADSCs in alginate and fibrin scaffolds. Methods: Adipose-derived stem cells were isolated from adipose tissue and cultured in alginate or fibrin scaffolds with a medium containing PRP 10% or FBS 10%. Then, the cell viability percentage was assessed by MTT assay and trypan blue staining. Also, the percentages of living, apoptotic, and necrotic cells were assessed by flow cytometry assay on the fourth and eighth days. Results: The cell viability rate was significantly higher in the fibrin scaffold group with PRP than in other groups on the fourth and eighth days (P < 0.05). Moreover, the rate of necrotic cells was significantly lower in the fibrin scaffold group than in the other groups (P < 0.05). Besides, the percentage of living cells was significantly higher in the fibrin scaffold group with PRP than in the other groups on the fourth and eighth days (P < 0.05). Also, the percentage of early apoptotic cells was significantly lower in fibrin with PRP than in other groups on the fourth day. There was no significant difference in the rate of late apoptotic cells between the groups (P > 0.05). Conclusions: These findings indicate the positive effect of PRP on the survival and proliferation of ADSCs compared with FBS. Therefore, PRP can be considered a suitable supplement to replace animal sera like FBS.