Abstract
Plackett-Burman design was used to efficiently select important medium components affecting the lipase production by Aspergillus niger using shea butter cake as the main substrate. Out of the eleven medium components screened, six comprising of sucrose, (NH4)2SO4, Na2HPO4, MgSO4, Tween-80, and olive oil were found to contribute positively to the overall lipase production with a maximum production of 3.35 U/g. Influence of tween-80 on lipase production was investigated, and 1.0% (v/w) of tween-80 resulted in maximum lipase production of 6.10 U/g. Thus, the statistical approach employed in this study allows for rapid identification of important medium parameters affecting the lipase production, and further statistical optimization of medium and process parameters can be explored using response surface methodology.
Highlights
Lipases (E.C. 3.1.1.3) are enzymes that primarily catalyze the hydrolysis of triacylglycerols and show enormous potentials in catalyzing the synthesis of esters through transesterification, thioesterification, and aminolysis in nonaqueous media [1, 2]
This study involves the use of Shea butter cake (SBC) as the solid substrate to identify some of the nutritional components contributing to lipase production by A. niger
This is due to the fact that oil cakes are among the agricultural residues commonly used for lipase production, since they contain some residual nutrients that can serve as both carbon and inducer sources, and have been reported to be good substrates for microbial enzyme production [19]
Summary
Lipases (E.C. 3.1.1.3) are enzymes that primarily catalyze the hydrolysis of triacylglycerols and show enormous potentials in catalyzing the synthesis of esters through transesterification, thioesterification, and aminolysis in nonaqueous media [1, 2]. Lipases have been utilized in different industrial processes including detergent formulation, flavor enhancement, treatment of fatty effluents, production of biosurfactants, biopharmaceutical formulations, and biodiesel production [3, 4]. Agroindustrial residues are continuously being generated in vast quantity especially in developing countries and their disposal is associated with several environmental problems [5]. Utilization of these agroresidues and byproducts of agroindustries as nutrient sources for microbial lipase production may reduce the final enzyme production cost, which is one of the major challenges affecting the large-scale production [6, 7]. Microbial lipases have been extensively studied due to their interesting characteristics such as stability in organic solvents, action under mild conditions, and high substrate specificity [2, 10]
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