Abstract
Objective To investigate the application of PCR-RFLP and PCR-SSCP in the inspection of foodborne pathogens. Methods 23S rRNA gene of 14 kinds of common foodborne pathogens were set as target genes, universal primers PCR (UP-PCR) was used in amplification, and restriction fragment length polymorphism (RFLP) analysis and single strand conformation polymorphism (SSCP) analysis were used to analyze PCR products digested fragments. Results RFLP for Hpa II digestion products 23S rRNA gene fragment analysis showed that RFLP pattern of different species of bacteria had similar performance; SSCP analysis showed that SSCP patterns had greater variability, so could provide an effective basis for the identification of bacteria. Conclusion PCR-SSCP technique can rapidly identify foodborne pathogens, so is worthy of promotion and application in the clinical. Key words: PCR-RFLP; PCR-SSCP; Foodborne pathogens; Inspection; Application
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