Abstract
Purpose: To optimize the extraction technology of polysaccharides from Cynomorium songaricum Rupr by ultrasonic-assisted extraction (UAE). Methods: Four parameters including ultrasonic power, ratio of raw material to water, extraction temperature, and extraction time were optimized by orthogonal design. The effects of the factors on the yield of polysaccharides were also studied. The hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were determined in vitro by spectrophotometry. Results: The optimal conditions were as follows: 1:30 as ratio of raw material to water, extraction for 70 min at 80 o C with ultrasonic power being 420 W. Under these conditions, the yield of polysaccharides was up to 4.51%, which was significantly higher than that obtained under the initial conditions (3.82 %). DPPH radical scavenging activity reached 64.82 % at 0.012 mg/mL, while hydroxyl radical scavenging activity was 18.36 % at 0.5 mg/mL. Conclusion: Ultrasonic-assisted extraction technology is a useful tool for the extraction of bioactive components from biological materials. Keywords: Cynomorium songaricum , Polysaccharide, Ultrasonic-assisted Extraction, Orthogonal Design
Highlights
Cynomorium songaricum Rupr. (Cynomoriaceae) is an achlorophyllous holoparasite with distribution in northwestern China
The effects of different ultrasonic powers on the yield of polysaccharides were investigated in the .commended study
We found that the ultrasonic power and extraction temperature were significant for the polysaccharide extraction from C. songaricum at the 99 % confidence level
Summary
Cynomorium songaricum Rupr. (Cynomoriaceae) is an achlorophyllous holoparasite with distribution in northwestern China. (Cynomoriaceae) is an achlorophyllous holoparasite with distribution in northwestern China. According to the ancient Chinese medical literature, C. songaricum is effective in regulating endocrinopathy and heightening sexual function and anti-aging such as dementia [3,4]. The research which emphases on the modern medicine and functionalized food chemistry mainly focuses on the physiological activities and structures of polysaccharides. A large number of researches on extraction technologies of various polysaccharides have been reported, including solvent extraction [11], biological enzymatic method [12] and ultrasonic extraction [13,14]
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