Application of Nucleic Acid Amplification Technology in Screening Blood Donors in Yancheng of Jiangsu Province

Abstract

Objective To explore the feasibility and necessity of the application of nucleic acid amplification technology (NAT) in screening blood donors in Yancheng.Methods From July 4th,2011 to July 3rd,2012,a total of 34 925 blood samples which were negative in enzyme-linked immunosorbent assay (ELISA) and qualified in alanine aminotransferase (ALT) detection,were sele.cted in this study stochastically.All the samples were detected hepatitis B virus (HBV)/ hepatitis C virus / human immunodeficiency virus by Roche Cobas S201 System in 6 samples pooling.NAT positive samples were reconfirmed by National Center for Clinical Laboratories (NCCL).Results The results showed that 57 samples in 34 925 serological negative specimens were NAT positive,and the rate of positive detection was 0.16 ％.Twenty of them were reconfirmed by NCCL and 13 were conformed HBV positive presented a viral load below 20 IU/mL.Of the 13 reconfirmed HBV positive samples,9 samples were occult hepatitis B (OBI),2 samples were ELISA omission.Conclusions ELISA could miss some virus,and occult infection of HBV are exist in local blood donors.NAT could be the supplement to the ELISA to improve the safety of local clinical blood use. Key words: Hepatitis B virus; Hepatitis C virus; Human immunodeficiency virus; Nucleic acid amplification technology