Abstract
A simple and rapid method of separation and quantification of five bile acids such as: cholic acid (C), deoxycholic acid (DC), chenodeoxycholic acid (CDC), lithocholic (LC) and ursodeoxycholic (UDC) with the use of the NP-TLC method has been developed. The five bile acids were satisfactory separated on aluminum and glass plates precoated with silica gel 60 and silica gel 60F254 (E. Merck, Art. 1.05553, Art. 1.05554, and Art. 1.05715) using mobile phase n-hexane–ethyl acetate–acetic acid in different volume compositions. For the optimal conditions of separation the quantitative analysis of studied acids was elaborated on. The quantitative analysis was provided with the use of densitometric detection at λ = 360 nm. Densitometric analysis has been used to find the linear correlations between the peak area and amount of each bile acid spotted on the chromatographic plates. The statistical parameters of obtained linear regressions indicated that NP-TLC method with densitometric detection is statistically significant and can be used for separation and detection of C, DC, CDC, LC, and UDC in biological samples and also in pharmaceutical formulations.
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