Abstract
Nextera™ technology is fast becoming the method of choice for the preparation of second-generation DNA-sequencing libraries. Other library-preparation workflows are limited by multistep protocols, sample loss, lack of automation capability, and labor costs. Nextera technology counters many of these bottlenecks and offers a simplified procedure applicable to multiple second-generation sequencing platforms. Here we describe the adaptation of the Nextera library-preparation procedure to RNA-seq library preparation.
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