Abstract
BackgroundAneuploidy is a leading cause of repeat implantation failure and recurrent miscarriages. Preimplantation genetic screening (PGS) enables the assessment of the numeral and structural chromosomal errors of embryos before transfer in patients undergoing in vitro fertilization. Array comparative genomic hybridization (aCGH) has been demonstrated to be an accurate PGS method and in present thought to be the gold standard, but new technologies, such as next-generation sequencing (NGS), continue to emerge. Validation of the new comprehensive NGS-based 24-chromosome aneuploidy screening technology is still needed to determine the preclinical accuracy before it might be considered as an alternative method for human PGS.ResultsIn the present study, 43 human trophectoderm (TE) biopsy samples and 5 cytogenetically characterized cell lines (Coriell Cell Repositories) were tested. The same whole genome amplified product of each sample was blindly assessed with Veriseq NGS and Agilent aCGH to identify the aneuploidy status. The result showed that the NGS identified all abnormalities identified in aCGH including the numeral chromosomal abnormalities (again or loss) in the embryo samples and the structural (partial deletion and duplication) in the Coriell cell lines. Both technologies can identify a segmental imbalance as small as 1.8 Mb in size. Among the 41 TE samples with abnormal karyotypes in this study, eight (19.5 %) samples presented as multiple chromosome abnormalities. The abnormalities occurred to almost all chromosomes, except chromosome 6, 7, 17 and Y chromosome.ConclusionsGiven its reliability and high level of consistency with an established aCGH methodology, NGS has demonstrated a robust high-throughput methodology ready for extensive clinical application in reproductive medicine, with potential advantages of reduced costs and enhanced precision. Then, a randomized controlled clinical trial confirming its clinical effectiveness is advisable to obtain a larger sequencing dataset and more evidence for the extensive use of NGS-based PGS.
Highlights
Aneuploidy is a leading cause of repeat implantation failure and recurrent miscarriages
The present study investigated the accuracy of next-generation sequencing (NGS) technology for comprehensive chromosome screening as a preclinical step before its clinical application in the diagnosis of chromosomal aneuploidy on embryos at blastocyst stage
whole genome amplification (WGA) is necessary for both Array comparative genomic hybridization (aCGH) and NGS, and it took three hours to process WGA
Summary
Aneuploidy is a leading cause of repeat implantation failure and recurrent miscarriages. Preimplantation genetic screening (PGS) enables the assessment of the numeral and structural chromosomal errors of embryos before transfer in patients undergoing in vitro fertilization. Validation of the new comprehensive NGS-based 24-chromosome aneuploidy screening technology is still needed to determine the preclinical accuracy before it might be considered as an alternative method for human PGS. Zheng et al Molecular Cytogenetics (2015) 8:38 aneuploidy screening of embryos derived from patients undergoing IVF, termed preimplantation genetic screening (PGS), enables the assessment of the numeral and structural chromosomal constitution of embryos before transfer. It has been applied to treat patients with increased risk for aneuploid embryos, and introduced into clinical practice to improve the chance of healthy conceptions after infertility treatment with poor prognoses, such as advanced maternal age, repeated implantation failure, and recurrent miscarriage [8, 9]. A latest research reviewed literatures on PGS for aneuploidy with analysis of all chromosomes showed that embryo implantation rates could be significantly increased by the transfer screened euploid embryos [10]
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