Abstract
Fusarium is a common fungal disease in grains that reduces the yield of barley and wheat. In this study, a near infrared reflectance spectroscopic technique was used with a statistical prediction model to rapidly and non-destructively discriminate grain samples contaminated with Fusarium. Reflectance spectra were acquired from hulled barley, naked barley, and wheat samples contaminated with Fusarium using near infrared reflectance (NIR) spectroscopy with a wavelength range of 1175–2170 nm. After measurement, the samples were cultured in a medium to discriminate contaminated samples. A partial least square discrimination analysis (PLS-DA) prediction model was developed using the acquired reflectance spectra and the culture results. The correct classification rate (CCR) of Fusarium for the hulled barley, naked barley, and wheat samples developed using raw spectra was 98% or higher. The accuracy of discrimination prediction improved when second and third-order derivative pretreatments were applied. The grains contaminated with Fusarium could be rapidly discriminated using spectroscopy technology and a PLS-DA discrimination model, and the potential of the non-destructive discrimination method could be verified.
Highlights
Barley (Hordeum vulgare L.) is one of the four major grains, and is the most important crop after rice in South Korea
The control group and experimental group of the hulled barley, naked barley, and wheat samples were classified by the first culture experiment results
In the raw pretreatment to to thethe rawraw reflectance spectra acquired fromfrom the hulled barleybarley samples
Summary
Barley (Hordeum vulgare L.) is one of the four major grains, and is the most important crop after rice in South Korea. It is mainly used for mixed rice, barley tea for children, and various organic food materials [1]. Barley has many functional nutrients such as β-glucan, arabinoxylan, and hydrocyanic acid, which other general grains do not have, and has effects that aid in the prevention of adult diseases, such as heart disease (by lowering the blood cholesterols) and obesity (by inhibiting body fat accumulation) [2,3]. Barleys can be generally classified into hulled barley, which is not peeled and is used as a feed, and naked barley, which is peeled and can be used for food [4].
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