Abstract
The fluorescent NBD group has come a long way in terms of biological applications since its discovery a few decades back. Although the field of fluorescently labeled lipids has grown over the years with the introduction of new fluorescent labels, NBD-labeled lipids continue to be a popular choice in membrane and cell biological studies due to desirable fluorescence characteristics of the NBD group. In this chapter, we discuss the application of NBD-labeled lipids in membrane and cell biology taking representative examples with specific focus on the biophysical basis underlying such applications.
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