Application of Natural Deep Eutectic Solvents in the Extraction of Quercetin from Vegetables.

Yunliang Dai,Kyung Ho Row

doi:10.3390/molecules24122300

Abstract

Quercetin is a phytochemical with disease prevention and health promotion activities that has attracted significant research attention. In this study, choline chloride and betaine-based natural deep eutectic solvents were prepared using a heating method. Their physical and chemical properties were also tested. Then, they were applied to extract quercetin from onion and broccoli with ultrasonic-assisted solid liquid method coupled with HPLC. Three factors (temperature, amount, and time) were considered for the optimization of the extraction assays. In the optimal conditions, the extraction recoveries were 88.91–98.99%, 88.45–99.01%, and 89.56–98.74% for quercetin, isorhamnetin, and kaempferol. Tailor-made natural deep eutectic solvents could be applied as sustainable and safe extraction media for biochemical applications.

Highlights

Quercetin (3, 5, 7, 30, 40 -pentahydroxyflavone), a polar polyphenolic compound with a molecular formula of C15 H10 O7, is a natural flavonoid found abundantly in many edible vegetables [1,2].Quercetin has attracted considerable attention because of its biologically important activities, such as anti-oxidant activity [3], anti-obesity effects [4], and cancer treatment potential [5,6]
The peak at cm−1 was attributed to alkyl group, and the -CH2 being vibration at 1480 cm−1 was a prominent group detected in all choline chloride (ChCl)-based Deep eutectic solvents (DESs)
natural deep eutectic solvents (NADESs) because of under the same conditions methanol as the extraction solvent, solid liquid extraction was operation applied to the extraction process of quercetin conditions with NADESs because of its as its high efficiency and simple

Summary

Introduction

Quercetin (3, 5, 7, 30 , 40 -pentahydroxyflavone), a polar polyphenolic compound with a molecular formula of C15 H10 O7 , is a natural flavonoid found abundantly in many edible vegetables [1,2].Quercetin has attracted considerable attention because of its biologically important activities, such as anti-oxidant activity [3], anti-obesity effects [4], and cancer treatment potential [5,6]. Deep eutectic solvents (DESs), generally tailor-made from two or three inexpensive and safe components that are capable of self-association, often through hydrogen bond interactions, to form a eutectic mixture with a melting point lower than that of each individual component. These solvents are new serial solvents or ionic liquid (IL) analogues that have been proposed to overcome some of the disadvantages of ionic solvents, including high melting point, large charge, and toxicity. DESs have been applied widely in areas, such as solvents [20], bioengineering [21,22,23], nanotechnologies [24], separation of natural compounds [25,26,27], and new materials [28,29,30]

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