Abstract
Caffeine of Molecularly Imprinted Polymer (MIP) has been synthesized by the cooling-heating method and to obtain sensor material to detect caffeine. The caffeine is verified through a potentiometric method based on galvanic cells involving anodes and cathodes. MIP of caffeine that has been made function as a cathode or working electrode and the modified of the Ag/AgCl reference electrode that made by reducing the AgCl membrane to nano size (44.45 nm) function as an anode. Then the modified Ag/AgCl reference electrode performance test is compared to the standard AgCl reference electrode. The result has shown the modified Ag/AgCl reference electrode produces linearity of the calibration curve in testing caffeine solutions with a concentration range of 0.1 ppm to 5.0 ppm. Based on the result of the modified Ag/AgCl reference electrode’s test range, we got the linearity coefficient value of 0.9993, with a slope of 0.9693 and an intercept of 0.1306. Generated accuracy calculation resulted in a value of 98.85% and a precision of 0.951%. The result of the lower limit of detection (LoD) was at a concentration of 0.2 ppm and the limit of quantification (LoQ) at a concentration of 0.4 ppm. Thus, it concluded that the modified Ag/AgCl reference electrode has the same or equivalent capability as a standard electrode.
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