Abstract
Application of Mycobacterial Peptide as Recombinant Antigen for Diagnosis of Bovine Tuberculosis
Highlights
Bovine tuberculosis (BTB) is a chronic infectious illness caused by an acid fact bacilli; Mycobacterium bovis (M. bovis), which infects cattle and other domesticated and wild animals (Michel et al, 2011)
Amplification of gene encoding early secretory antigenic target 6 (ESAT-6) A single band of 288 bp was successfully amplified from M. bovis genome represented the gene encoding ESAT-6 protein (Figure 1)
Construction of the expression vector; pQE30ES In our work,ESAT-6 gene was inserted into the prokaryotic vector pQE30, the clones were screened firstly using a set of restriction enzymes (REs); BgIII and BamHI
Summary
Bovine tuberculosis (BTB) is a chronic infectious illness caused by an acid fact bacilli; Mycobacterium bovis (M. bovis), which infects cattle and other domesticated and wild animals (Michel et al, 2011). This illness causes great negative economic impacts in livestock industry via condemnation of animal and animal products. In spite of its rate in developed countries is greatly minimized due to application of milk pasteurization (Hardie et al, 1992), the insistence of M. bovis still regards for up to 10% of the human cases. It is evident that more efficacious diagnostic techniques with boosted specificity for extirpation, and monitoring programs are demanded to control BTB (Picasso-Risso et al, 2021)
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