Application of multiplexing fiber optic laser induced fluorescence spectroscopy for detection of aflatoxin B1 contaminated pistachio kernels

Abstract

To explore the effect of signal acquisition way on screening ability, the multiplexing fiber optic laser induced fluorescence spectroscopy (LIFS) system with one-, two- and three-probe, were employed respectively to detect artificially aflatoxin B1 (AFB1, 5, 10, 20, 30, and 50 ppb) contaminated 300 pistachio kernels in this study. Compared to one- and two-probe modes, highest accuracy (≥97.0%) by support vector machine (SVM) employing 390–660 nm were obtained using three-probe, which also showed the most attractive precision (root mean square error of prediction (RMSEP) < 4.5 ppb) for AFB1 by stepwise multiple linear regression (SMLR) using 174–1100 nm. These suggested that the effective collection of spatial information could improve the performance of model, and the three-probe LIFS had a preliminary feasibility for discriminating pistachios contaminated with low concentration of AFB1. Further study on classifying naturally contaminated samples is needed to validate the applicability of this system.