Application of Morning Glory Flower (Ipomea purpurea) Extract for Colouring Plant Section Preparation

Siti Zaenab,Windi Kurniasari,Dewinta Hapsari Maharani,Sri Wahyuni,Ayu Iriani

doi:10.22236/j.bes/536738

Siti Zaenab, Windi Kurniasari + Show 3 more

Open Access

https://doi.org/10.22236/j.bes/536738

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Abstract

Background: Preparing requires colouring to clarify or sharpen various tissue parts, especially cells. The use of synthetic dyes in preparations in the long term can less impact both living things and the environment. The study aims to obtain natural dyes from morning glory flowers at optimal temperatures and pH and find out the feasibility of preparations coloured using Morning Glory flower extract. Method: The extraction process uses a maceration method with different temperature variations of 400C, 500C, 600C, 700C, 800C and different pH i.e. 3, 4, 5, 6. The preparation process uses different dye concentration variations. The concentrations used are 50%, 60%, 70%, 80%, 90%, 100%. Data analysis techniques use descriptive and percentage analysis methods. Results: Morning glory flower extract (Ipomoea purpurea (L.) Roth) can colour dermal tissue, empulur, cortex and transport beams on the stems of cayenne pepper plants (Capsicum frutescens L.). Conclusion: Natural dyes of morning glory flowers can be used as a learning medium with a 74% eligibility rate for preparing with morning glory flower dyes.

Highlights

The availability of quality plant tissue preparations becomes very important in microscopic study
This study included qualitative descriptive research, which described the utilization of morning glory flower extract
The study sample used Morning glory flowers that grow in rice fields and roadside Batu City which was used as a natural dye, and cayenne pepper stems (Capsicum frutescens L) obtained from the rice fields of Batu city, which were made preparing a plant section

Summary

Introduction

The availability of quality plant tissue preparations becomes very important in microscopic study. In preparations, colouring is needed to clarify or sharpen various tissue parts, especially in the cells. During this time, the manufacture of preparations is still very dependent on synthetic dyes. The use of carcinogenic synthetic dyes can cause skin allergies that can even develop into skin cancer resulting in environmental pollution (Tisnadjaja, 2014; Wilujeng & Kusnawati, 2010; Zulfiyah et al, 2015). This encourages attention to return to the utilization of natural dyes

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