Abstract
BackgroundThis study was aimed to explore the application value of modified closed biopsy technique in puncture biopsy of rabbit VX2 transplanted bone tumor model.MethodsVX2 tumor was transplanted into the bilateral tibia of 30 rabbits through the tibial plateau to make the model of VX2 transplanted bone tumor. Seven days after modeling, the proximal tibia biopsy was performed under the guidance of X-ray, and the biopsy specimen was examined pathologically. The left leg was biopsied with modified closed biopsy technique (experimental group), and the right leg was biopsied with hollow needle (control group). After 14 days of modeling, all rabbits were killed after X-ray examination around the puncture hole, and the soft tissue around the puncture hole was taken for pathological examination, and the expression levels of PCNA and CD34 in the tissue extract were detected by enzyme-linked immunosorbent assay (ELISA).ResultsBy the end of the experiment, a total of 3 rabbits died, and finally, 27 rabbits were included in the study. Tumor cells were detected in all the intramedullary specimens obtained by puncture biopsy. On the 14th day after modeling, X-ray showed that the occurrence rate of periosteal reaction and extraosseous high-density shadow around the puncture hole was 14.81% (4/27) in the experimental group and 40.74% (11/27) in the control group. The difference was statistically significant (P<0.05). The pathological results of soft tissue around the puncture hole showed that the tumor cell metastasis rate was 29.63% (8/27) in the experimental group and 100% (27/27) in the control group, and the difference was statistically significant (P<0.05). The expression levels of PCNA and CD34 in the experimental group were lower than those in the control group (P < 0.05).ConclusionBoth the modified closed biopsy technique and needle aspiration biopsy can provide sufficient biopsy tissue for the diagnosis of VX2-transplanted bone tumor in rabbits. At the same time, the improved closed biopsy technique has a certain application value in preventing local metastasis of tumor cells along the puncture channel.
Highlights
This study was aimed to explore the application value of modified closed biopsy technique in puncture biopsy of rabbit VX2 transplanted bone tumor model
Malignant bone tumors account for 6% of malignant tumors in adolescents under 20 years old, of which about 2/ 3 are osteosarcoma, and most of the others belong to Ewing sarcoma family [1]
Imaging and pathological examination results Fourteen days after modeling, X-ray examination results showed that periosteal reaction and high-density shadow around the tibial puncture hole appeared in 4 rabbits in the experimental group and 11 rabbits in the control group (Fig. 4)
Summary
This study was aimed to explore the application value of modified closed biopsy technique in puncture biopsy of rabbit VX2 transplanted bone tumor model. A large number of studies have shown that rabbit VX2 tumor grows rapidly and can be inoculated into the lung, liver, muscle, or bone tissue [6, 7]. Rabbit VX2 bone tumor model has the similar biological characteristics of human malignant bone tumor, which can be used to simulate the proliferation and metastasis process of human bone tumor [8, 9]. PCNA is a good indicator of cell proliferation, and CD34 is a characteristic surface marker of hematopoietic stem/progenitor cells. The purpose of this study was to investigate the application value of improved closed biopsy technique in puncture biopsy of VX2 bone tumor model in rabbits
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