Application of mineral carriers for immobilization of Trichoderma viride

Abstract

There is no doubt in the fact that preparations containing Trichoderma as the main component are currently a real alternative to synthetic agrochemicals as antagonists of soil-borne plant diseases and growth stimulators. The use of this kind of drugs does not always give sufficiently stable results. In addition, technological problems can often arise, which are associated with the short shelf life of liquid preparations based on Trichoderma. The use of the solid form of the drug often causes the problem of self-inhibition of conidia germination. And therefore a more careful calculation of the optimal dose for each specific strain is required. The development of multifunctional bio-preparations that are more stable in their effectiveness, especially preparations that include Trichoderma, is one of the most important tasks in the system of biotechnology development, which determined the relevance of the research. The aim of the work is to establish the optimal carrier for the immobilization of Trichoderma viride cells and the effect of immobilization on the stability of drugs. It has been experimentally established that the growth and development of the immobilized fungus Trichoderma viride is influenced by the nature and method of modification of the carrier. It was established that when the fungus immobilized on native and modified carriers (particle size 0.5 μm – 2.5 mm) was seeded on a sterile nutrient medium, a decrease in the growth rate of Trichoderma viride was found, compared to the control one where the native fungus was used. It has been proven that the optimal amount of grinding of both native and modified media is – 150 μm – 1.5 mm. It has been found that saponite modified with starch is the optimal carrier for Trichoderma viride immobilization. It has been proven that upon immobilization of the fungus Trichoderma viride, the latter becomes more resistant to the negative factors of the growth environment (antagonistic action of the natural conglomerate of microorganisms residing on spoiled alfalfa hay). Key words: fungi, cell immobilization, carriers, modified starch, humic acids, saponite, zeolite, zeolite-containing basalt tuff.