Abstract
In our experiment, the method of in situ hybridization with some modification was used to quickly screen out the transformed colonies of dinitrogen fixing filamentous cyaobacteria Anabaena azollae. In order to effectively complete lysis of A. azollae cells, which can cause colonies on nitrocellulose membrane over agar plate, several physical methods, such as freeze-thawing with liquid nitrogen, microwave treatment and incubation at
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