Application of micro-solid-phase extraction for determination of released dental fillings components in artificial saliva solution

Abstract

In this work, the microextraction devices in form of sachets were prepared and used for isolation of released dental fillings components from artificial saliva solutions As sorbents mesoporous aluminosilicate and C18-bonded silica were used. Gas chromatography was used for indication of analytes in samples. The dependence of adsorption on variable factors were determined. The effect of adsorbent mass, the membrane type for μ-SPE device and time of adsorption on the efficiency of the process were examined. The dependence between the extraction recovery and the desorption solvent was also indicated. Based on calculated adsorption capacities in time of adsorption and equilibrium state the kinetic study was prepared. The validation of determination method by using of membrane-protected microextraction for sample preparation was conducted. The developed method proved to be convenient and offers good reproducibility. The detection limits of the method under optimized conditions were in the range of 0,117 μg mL−1 for camphor to 1407 μg mL−1 for 4-methoxyphenol. Two examined membrane protected sorbent were successfully applied for extraction of eluted dental ingredients.