Abstract
Application of Macro-Synthetic-Fiber-Reinforced Sprayed Concrete for Primary Lining Support on Highway Tunnel Project in Bosnia and Herzegovina
Highlights
MethodsAvailable oral squamous cell carcinomas (SCC25, SCC15, CAL27) were used for this study
The programmed death ligand or PDL-1 has been shown to inhibit tumor immunology and facilitate immune evasion
The application of macro synthetic fiber reinforced sprayed concrete is seen by many design engineers as the alternative to steel reinforced sprayed concrete
Summary
Available oral squamous cell carcinomas (SCC25, SCC15, CAL27) were used for this study. RNA was isolated and subsequently screened using validated primers specific for B7-H1/PDL-1. RNA was quantified and screened for purity prior to normalization and the RT-PCR screening procedure. Results: mRNA was successfully isolated from each cell line and verified using spectrophotometric measurements and internal PCR control standards. Subsequent screening using validated primer sets revealed expression of B7-H1 in all oral cancer cell lines, with differential expression observed using the PDL-1 validated primer set. No expression was observed in the normal, non-cancerous oral gingival cell line examined
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