Application of luminescence spectral assay for assessment of the functional state of birds’ gastrointestinal tract by nucleic acids content in cells

Abstract

The method of functional state evaluation of chickens’ glandular stomach mucous membrane epithelium, using luminescence spectral analysis with fluorescent dye “Ethidium bromide” was proposed. The dynamics of nucleic acids content in in cells of chickens’ glandular stomach under experimental escherichiosis and in intact poultry was established using this method. A gradual increase in the nucleic acids content in epithelium of glandular stomach of intact chickens was revealed from 1th to 30th days. The nucleic acids content in cells of chickens under experimental escherichiosis was gradually increased from the fourth day of life (second day after infection). The minimal index of nucleic acids content was recorded at the 7th day of chickens’ life. From 8th to 30th days of life, periods of increase / decrease in nucleic acids quantity in the cells were noted. By the 30th day, nucleic acids content in the chickens glandular stomach epithelial cells of the experimental groups was less by 40.1% than in control group poultry.