Application of ligand-exchange capillary electrophorersis to the chiral separation of α-hydroxy acids and β-blockers

Abstract

The application of the principle of ligand-exchange capillary electrophoresis to two substance classes is described. As chiral selector N-(2-hydroxyoctyl)- l-4-hydroxyproline–copper(II) complex was used. This principle was applied to the chiral separation of α-hydroxy acids and drugs containing amino alcohol structure such as β-blockers. The enantioselectivity was found to be strongly dependent on pH corresponding to the optimal conditions for complex formation for each structure class.