Application of Lab-on-Chip for Detection of Microbial Nucleic Acid in Food and Environment.

Liu Yang,Liu Yang,Mengjiao Xu,Mengjiao Xu,Fangfang Sun,Fangfang Sun,Wei Yi,Wei Yi,Xiaoyue Bi,Yao Xie,Yao Xie,Yao Xie,Jianping Dong,Zhan Zeng,Zhan Zeng,Minghui Li,Minghui Li,Minghui Li

doi:10.3389/fmicb.2021.765375

Abstract

Various diseases caused by food-borne or environmental pathogenic microorganisms have been a persistent threat to public health and global economies. It is necessary to regularly detect microorganisms in food and environment to prevent infection of pathogenic microorganisms. However, most traditional detection methods are expensive, time-consuming, and unfeasible in practice in the absence of sophisticated instruments and trained operators. Point-of-care testing (POCT) can be used to detect microorganisms rapidly on site and greatly improve the efficiency of microbial detection. Lab-on-chip (LOC) is an emerging POCT technology with great potential by integrating most of the experimental steps carried out in the laboratory into a single monolithic device. This review will primarily focus on principles and techniques of LOC for detection of microbial nucleic acid in food and environment, including sample preparation, nucleic acid amplification and sample detection.

Highlights

Pathogenic microorganisms refer to any microorganism capable of injuring its host by competing with it for metabolic resources, destroying its cells or tissues, or secreting toxins
The microfluidic chip integrated with real-time fluorescence loop-mediated isothermal amplification technology developed by Zhou et al. (2021) has good sensitivity and specificity, but preprocessing of samples is required to enrich target DNA
The integrated chip designed by Park et al. (2018a) for the detection of Staphylococcus aureus and E. coli in food integrates nucleic acid amplification and electrochemical detection, and requires advance DNA extraction and purification

Summary

Introduction

Pathogenic microorganisms refer to any microorganism capable of injuring its host by competing with it for metabolic resources, destroying its cells or tissues, or secreting toxins. Microfluidic technology integrates sample preparation, nucleic acid amplification, and sample detection on a chip. The technology of nucleic acid amplification and sample detection has been well developed, and a variety of emerging experimental technologies have been timely designed and implemented on chip.

Results

Conclusion