Abstract
Ion chromatography procedure for the simultaneous determination of histamine, tyramine, phenylethylamine, cadaverine and putrescine in different food samples is described. The simple procedure based on isocratic elution, 5 mM HNO3 without organic modifier as mobile phase and conductometric detection without suppression was proposed and discussed in details. The linearity of the tested procedure was between 0.5–5 and 5–100 mg/L, average recoveries for the standard solutions of amines varied between 98–100%, whereas in standard addition method between 96–99%, that indicated acceptable accuracy of the discussed method. The satisfactory reproducibility was proved by values of coefficient of variations (CV) in the range 0.2–5.0% for all tested food samples. The proposed method reveals a number of advantages: fast elution of analytes without mobile phase conductivity suppression, lack of derivatization step, simple sample preparation and environmentally friendly character.
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