Abstract
The present study explored the use of internal transcribed spacers (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA (nrDNA) for identification of Echinops mandavillei Kit Tan, an endemic species to Saudi Arabia. The sequence similarity search using Basic Local Alignment Search Tool (BLAST) and phylogenetic analyses of the ITS sequence of E. mandavillei Kit Tan showed high level of sequence similarity (98%) with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. mandavillei could have a potential use for molecular genotyping.DOI: http://dx.doi.org/10.3329/bjpt.v21i1.19256Bangladesh J. Plant Taxon. 21(1): 33-42, 2014 (June)
Highlights
The genus Echinops L. belonging to the subtribe Echinopsinae of Cynareae, of the family Asteraceae comprise about 120 species (Vidović, 2011), and distributed in tropical Africa, the Mediterranean basin, temperate regions of Eurasia, Central Asia, Mongolia and North-eastern China, with the maximum number of species occurring in the Caucasus and the Middle East (Susanna and Garcia-Jacas, 2007)
E. abuzinadianus, E. mandavillei and E. sheilae are endemic to Saudi Arabia, while remaining species have been reported from different geographic locations of Arabian Peninsula
The internal transcribed spacers (ITS) region (ITS1-5.8S-ITS2) of Echinops mandavillei Kit Tan sequenced in the present study was found 634 bp, where ITS1 region 252 bp (GC content 54%), 5.8S gene 164 bp (GC content 53%), and ITS2 region 218 bp (GC content 50%)
Summary
The genus Echinops L. belonging to the subtribe Echinopsinae of Cynareae, of the family Asteraceae comprise about 120 species (Vidović, 2011), and distributed in tropical Africa, the Mediterranean basin, temperate regions of Eurasia, Central Asia, Mongolia and North-eastern China, with the maximum number of species occurring in the Caucasus and the Middle East (Susanna and Garcia-Jacas, 2007). E. yemenicus Kit Tan. Of them, E. abuzinadianus, E. mandavillei and E. sheilae are endemic to Saudi Arabia, while remaining species have been reported from different geographic locations of Arabian Peninsula. The DNA sequence technology provides series of new data for molecular phylogeny and DNA barcoding which has now-a-days changed the paradigm of species identification (Ali and Choudhary, 2011; Ali et al, 2014).
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