Abstract
Cells ofThermoanaerobium brockii were immobilized by entrapment methods as easy-to-handle biocatalyst for stereoselective reductions of oxo-acid esters. Different matrix materials were tested: agarose, k-carrageenan, alginate, polyacrylamide and polyurethanes. The two latter matrices allowed useful lifetimes of the immobilized biocatalysts of more than 2 months at thermophilic operation temperatures (around 65°C). Permeabilization of cells did not improve the catalytic activity. Immobilization of the cells did not enhance the thermostability. Only after a considerable period of operation could the immobilized biocatalysts be fed with medium lacking the complex substrates yeast extract and tryptone. Compared with freely suspended cells, reaction rates were lower.
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