Abstract
Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.
Highlights
Burns are tissue damage or loss that are caused by contact with very high temperatures, chemicals, electricity, or radiation (Yefta, 2009)
Day-7 observation showed significant differences in the average wound area between treatment groups. (Table 1) Significant difference in average wound area occurred between Human adipose derived stem cells (hADSC) in collagen gel treatment group with the smallest wound area compared to control group (Tabel 3)
At day-14 and -21, rats that were given hADSC in collagen gel showed the smallest wound area compared to the other treatment groups (Fig. 3 and 4) and the average wound area was significantly different between groups, where the average wound area of hADSC in collagen gel treatment group was the smallest (Table 4 and 5)
Summary
Burns are tissue damage or loss that are caused by contact with very high temperatures, chemicals, electricity, or radiation (Yefta, 2009). Complications from improper handling of burn wounds can produce scars that are tense, protruding and forming fibrotic bands that lose elasticity. In wounds that experience spontaneous reepithelialization in less than ten days, the incidence of hypertrophic scarring and contracture is four percent. In wounds that experience spontaneous re-epithelialization are more than twenty day, hypertrophic scarring and contracture incidence increased almost twenty-fold (7580%). Wound management refers to wound closure procedure with surgical intervention if the wound is estimated to experience spontaneous re-epithelialization in more than twenty-one days (Yefta, 2009; 2016)
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