Application of HPV prevalence and viral load in cervical lesions in Shanxi

Abstract

Objective To study the prevalence of human papillomavirus (HPV) in Shanxi province, and to investigate the relationship between HPV viral load and cervical cancer progression. Methods Real time fluorescence quantitative PCR was used to detect the copy number of HPV DNA copies, the cervical cells of HPV 16 and HPV 18 positive patients were detected by thin-layer cytologic test (TCT), the Bethesda system wasperformed. Results A total of 15 518 women were enrolled in our study, 3 199 (20.61%) of them were identified as positive. The most common HPV types were HPV l6, HPV52 and HPV58. The viral loads of normal/LSIL CIN1, 2/3 CIN CIN and cervical cancer (CC) group for HPV16 were 3.13 (2.48 - 3.74), 4.48 (4.10 - 5.14), 4.10 (4.41 - 5.63) log copies / 104 cells, the viral load of CIN2 / CIN 3 group was higher than that of the normal/LSIL/CIN1 group (P<0.01), but was lower than that of CC group (P<0.01). The viral load for HPV18 were 4.10 (2.86-4.88), 3.90 (2.93-5.31), and 3.94 (3.57-4.73) log copies/104 cells respectively, and there was no significant difference (P=0.983) among the three groups. Conclusions HPVI6 subtype viral load in cervical lesions is correlated with cervical lesions, the HPV viral load in the unit cells of the lesion increase with the increase of cervical lesions, the load of HPV18 subtype is not related to the degree of cervical lesions. Key words: Human papillomavirus; Viral load; Cervical cancer