Abstract
Liquid Chromatography and Fourier Transform Infrared Spectroscopy for quantitative analysis of individual and total curcuminoid in Curcuma longa extractRatna Wulandari, Sudjadi, Sudibyo Martono, Abdul Rohman
Highlights
The use of temula wak, known as Java turmeric, with scientific name Curcuma xanthorriza as raw material in herbal medicines and food is increased 5.4% annually, which make high demand on this rhizome in herbal industries (Nihayati et al, 2013)
The effectiveness of herbal medicine containing Curcuma extract depends on the active compounds of curcumin (CUK) and desmethoxycurcumin (DMCUR) present in formulation
The main component present in rhizome of C. xanthorriza is curcuminoid, believed to be responsible for those biological activities, it is important to determine the levels of compounds or groups of compounds responsible for biological activities
Summary
The use of temula wak, known as Java turmeric, with scientific name Curcuma xanthorriza as raw material in herbal medicines and food is increased 5.4% annually, which make high demand on this rhizome in herbal industries (Nihayati et al, 2013). Several biological activities have been reported in C. xanthorriza for treatment of hepatitis, liver complaints, diabetes, cancer, rheumatism, hypertension, antioxidant, diuretic, and hepatoprotective effects (Devaraj et al, 2014). Several analytical methods have been developed and used for routine analysis of curcuminoid, namely Fourier transform infrared spectroscopy (Tanaka et al, 2008; Rohman et al, 2015), thin layer chromatography-densitometry (Péret-Almeida et al., 2005), high performance liquid chromatography using UV-visible detector and electrochemistry detector (Inoue et al, 2008; Syed et al, 2015), high performance liquid chromatography using
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