Abstract
The SSF lipase production from Penicillium simplicissimum using an agro industrial residue and the biosurfactant from Pseudomonas aeruginosa were employed for a combined pre-treatment of wastewater from poultry processing industry. During the hydrolysis step of the wastewater fat better results were obtained at 34°C with biosurfactant 0.4% (v/v) and liquid enzyme preparation 6.2% (v/v) or at 46°C with biosurfactant 0.1% (v/v) and liquid enzyme preparation 3.8% (v/v). The pre-hydrolysis in two different conditions of enzyme and biosurfactant concentrations at 34°C and posterior anaerobic treatment allowed the COD removal and methane production, while the no hydrolyzed wastewater besides the COD removal, did not show methane production. Those results suggest the importance and efficiency of the pre-treatment with home-made lipases and biosurfactant during the high fat content wastewater pre-treatment.
Highlights
In Brazil, poultry slaughtering industry stands out as one of the most important industrial activities
Considering the need to promote the proper treatment of wastewater with high fat content, this paper aims the use of an extracellular lipase from Penicillium simplicissimum produced by Solid State Fermentation (SSF) in babassu cake and a rhamnolipid produced by Pseudomonas aeruginosa PA1 and its application in wastewater pre-treatment from a poultry processing industry
We investigated the kinetic of hydrolysis with two different amounts of liquid enzyme preparation (LEP) (1 and 5% v/v) at 30°C, 400 rpm
Summary
In Brazil, poultry slaughtering industry stands out as one of the most important industrial activities. Brazil is the third largest chicken’s producer, reaching 11 million tons of chicken meat produced, with 3.6 million for export [1]. The poultry slaughtering generate high volumes of effluent containing biodegradable organic matter, lipids, proteins and cellulose. Lipids can represent over 67% of the COD particulate in slaughterhouses wastewater [2]. With demand for energy, the search for alternative energy sources is increasing. The anaerobic processes involving as effluents generated in the food industry become advantageous, since they allow the energy production in form of methane gas [3]
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