Abstract
Ion-exchange and reversed-phase high performance liquid chromatographic methods have been developed and applied to the analysis of metabolites related to the biosynthesis of natural polyamines some amino acids and nucleotides in P388 leukemia cells. Key-enzyme activities concerning the ornithine utilization were measured by quantitating dansylated putrescine and citrulline. Thus the efficacy of enzyme-targeted chemotherapy using DL-α-difluoromethylornithine as inhibitor of ornithine-utilizing enzymes could be monitored. A marked increase of uridine-5′ and cytidine-5′ phosphates induced by DL-α-difluoromethylornithine treatment was demonstrated in P388 leukemia cells using ion-pair and ion-exchange liquid chromatographic methods.
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