Abstract
Objectives: This study aimed to develop and validate a simple, rapid, economical, precise and accurate HPTLC method for simultaneous determination of ofloxacin and racecadotril has been developed. Methods: Chromatographic separation was achieved using silica gel aluminum plate 60 F254 (10*10) as a stationary phase and dichloromethane: methanol: tri-ethylamine (95:5:0.1 by volume) as a mobile phase. The developed plates scanned densitometrically using UV detector. Detection was carried out at 254 nm over the concentration range of 100-800 ng/spot for ofloxacin and 30-240 ng/spot for racecadotril. The Rf value of ofloxacin and racecadotril was found to be 0.15 and 0.85 respectively. Results: The method is validated for different validation parameter such as linearity, accuracy, precision, LOD, LOQ and robustness and the result were found to be within the acceptance limit as per the guideline of international conference on harmonization (ICH). Conclusion: The described TLC-densitometric method was successfully applied to simultaneous determination of ofloxacin and racecadotril in their pure forms and pharmaceutical dosage form without previous separation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
