Abstract
The aim of this work was to study the application of genetic algorithm (GA) in modelling and optimization of cellulose production by Trichoderma reesei from pea hull. Enzyme activity of cellulase was determined using Filter Paper Activity (FPA) assay. Optimization of process parameters was performed using mathematical (MO) and genetic optimizers to obtain combination of variables for highest possible enzyme activity. GA generated a higher value of cellulase activity (0.353 U/mL) as compared to MO (0.302 U/mL). The values of independent variables in set (GA, MO) were: agitation speed (127, 120 rpm), %H2O2 concentration (10.36, 5.0), cultivation time (112, 91 h). The investigation highlights that GA could be used as a potential optimizer for processes involving waste utilization.
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