Abstract
We demonstrate successful applicability of fused–core column in analysis of biological samples (diluted urine and SPE–pre-purified plasma or serum) using 2 dimensional chromatography. Implementation of fused-core material in the second dimension of 2D chromatographic platform allowed improving S/N above 4 fold in comparison to traditional porous C18 column. Sample load onto the first dimension (5 μm) eliminates limitations of sample volume to be injected. The main limitation factor of the fused-core material–low loading capacity was overcome by using 2D chromatography. We emphasize importance of reduction of post-column (extra column) volume in LC/MS applications utilizing fused-core columns.
Highlights
Fused core particle in 1D chromatographyFused-core particle columns are a growing field
We report an on-line two-dimensional (2D) column switching method aimed to minimize the drawbacks of fused-core particle columns applied to complex biological samples
Transfer of the analyte from the first dimension to the second UHPLC dimension can only be accomplished via online collection loop(s) [9], because trap column(s) between the first and second dimension, as well as the first dimension column, are not designed to tolerate such high pressures, generated by the second dimension
Summary
Fused core particle in 1D chromatographyFused-core particle columns are a growing field. The relatively low pressure generated by fused core particles and the thin (about 0.5 μm) layer of stationary phase allows operation of fast flow rates without the loss of resolution by using standard HPLC equipment. We report an on-line two-dimensional (2D) column switching method aimed to minimize the drawbacks of fused-core particle columns applied to complex biological samples.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
